splenocyte complete medium Search Results


splenocyte medium  (ZeptoMetrix corporation)
94
ZeptoMetrix corporation splenocyte medium
Figure 1. (Continued) G, XTT assay for 4T1 cancer cell proliferation following supplementation of the medium with UC intermediates (n = 3, two-way ANOVA). P values: 24 hours: aspartate: not significant, ammonia = 0.007, glutamate and fumarate <0.0001; 48 and 72 <0.0001 for all measurements. Metabolite concentrations were supplemented in the following concentrations: ammonia, 0.75 mmol/L as published (49); aspartate, 0.25 mmol/L; fuma- rate, 0.35 mmol/L; glutamate, 0.25 mmol/L. H, Ex vivo FACS analysis of CD8+ <t>splenocyte</t> cell survival (left) and activation (right) following supplementa- tion with 1 mmol/L ammonia (Amn; ref. 59; measured plasma ammonia levels 1.34 mmol/L; n = 5, Student t test); P = 0.011, 0.043, respectively. I, Heat map for differential gene expression in hepatocytes demonstrates a unique pattern on day 21 in the livers of 4T1 breast cancer mice compared with day 4. J, 4T1 day 21 vs. 4T1 day 4 pathway enrichment analysis. Each bar shows the fold enrichment of a specific pathway. TCA, tricarboxylic acid.
Splenocyte Medium, supplied by ZeptoMetrix corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/splenocyte medium/product/ZeptoMetrix corporation
Average 94 stars, based on 1 article reviews
splenocyte medium - by Bioz Stars, 2026-02
94/100 stars
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splenocyte complete medium  (Thermo Fisher)
99
Thermo Fisher splenocyte complete medium
Figure 1. (Continued) G, XTT assay for 4T1 cancer cell proliferation following supplementation of the medium with UC intermediates (n = 3, two-way ANOVA). P values: 24 hours: aspartate: not significant, ammonia = 0.007, glutamate and fumarate <0.0001; 48 and 72 <0.0001 for all measurements. Metabolite concentrations were supplemented in the following concentrations: ammonia, 0.75 mmol/L as published (49); aspartate, 0.25 mmol/L; fuma- rate, 0.35 mmol/L; glutamate, 0.25 mmol/L. H, Ex vivo FACS analysis of CD8+ <t>splenocyte</t> cell survival (left) and activation (right) following supplementa- tion with 1 mmol/L ammonia (Amn; ref. 59; measured plasma ammonia levels 1.34 mmol/L; n = 5, Student t test); P = 0.011, 0.043, respectively. I, Heat map for differential gene expression in hepatocytes demonstrates a unique pattern on day 21 in the livers of 4T1 breast cancer mice compared with day 4. J, 4T1 day 21 vs. 4T1 day 4 pathway enrichment analysis. Each bar shows the fold enrichment of a specific pathway. TCA, tricarboxylic acid.
Splenocyte Complete Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/splenocyte complete medium/product/Thermo Fisher
Average 99 stars, based on 1 article reviews
splenocyte complete medium - by Bioz Stars, 2026-02
99/100 stars
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glutamax  (Thermo Fisher)
90
Thermo Fisher glutamax
Figure 1. (Continued) G, XTT assay for 4T1 cancer cell proliferation following supplementation of the medium with UC intermediates (n = 3, two-way ANOVA). P values: 24 hours: aspartate: not significant, ammonia = 0.007, glutamate and fumarate <0.0001; 48 and 72 <0.0001 for all measurements. Metabolite concentrations were supplemented in the following concentrations: ammonia, 0.75 mmol/L as published (49); aspartate, 0.25 mmol/L; fuma- rate, 0.35 mmol/L; glutamate, 0.25 mmol/L. H, Ex vivo FACS analysis of CD8+ <t>splenocyte</t> cell survival (left) and activation (right) following supplementa- tion with 1 mmol/L ammonia (Amn; ref. 59; measured plasma ammonia levels 1.34 mmol/L; n = 5, Student t test); P = 0.011, 0.043, respectively. I, Heat map for differential gene expression in hepatocytes demonstrates a unique pattern on day 21 in the livers of 4T1 breast cancer mice compared with day 4. J, 4T1 day 21 vs. 4T1 day 4 pathway enrichment analysis. Each bar shows the fold enrichment of a specific pathway. TCA, tricarboxylic acid.
Glutamax, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/glutamax/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
glutamax - by Bioz Stars, 2026-02
90/100 stars
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splenocyte complete medium  (Cambrex)
90
Cambrex splenocyte complete medium
Figure 1. (Continued) G, XTT assay for 4T1 cancer cell proliferation following supplementation of the medium with UC intermediates (n = 3, two-way ANOVA). P values: 24 hours: aspartate: not significant, ammonia = 0.007, glutamate and fumarate <0.0001; 48 and 72 <0.0001 for all measurements. Metabolite concentrations were supplemented in the following concentrations: ammonia, 0.75 mmol/L as published (49); aspartate, 0.25 mmol/L; fuma- rate, 0.35 mmol/L; glutamate, 0.25 mmol/L. H, Ex vivo FACS analysis of CD8+ <t>splenocyte</t> cell survival (left) and activation (right) following supplementa- tion with 1 mmol/L ammonia (Amn; ref. 59; measured plasma ammonia levels 1.34 mmol/L; n = 5, Student t test); P = 0.011, 0.043, respectively. I, Heat map for differential gene expression in hepatocytes demonstrates a unique pattern on day 21 in the livers of 4T1 breast cancer mice compared with day 4. J, 4T1 day 21 vs. 4T1 day 4 pathway enrichment analysis. Each bar shows the fold enrichment of a specific pathway. TCA, tricarboxylic acid.
Splenocyte Complete Medium, supplied by Cambrex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/splenocyte complete medium/product/Cambrex
Average 90 stars, based on 1 article reviews
splenocyte complete medium - by Bioz Stars, 2026-02
90/100 stars
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complete rpmi1640 culture medium  (Thermo Fisher)
90
Thermo Fisher complete rpmi1640 culture medium
Figure 1. (Continued) G, XTT assay for 4T1 cancer cell proliferation following supplementation of the medium with UC intermediates (n = 3, two-way ANOVA). P values: 24 hours: aspartate: not significant, ammonia = 0.007, glutamate and fumarate <0.0001; 48 and 72 <0.0001 for all measurements. Metabolite concentrations were supplemented in the following concentrations: ammonia, 0.75 mmol/L as published (49); aspartate, 0.25 mmol/L; fuma- rate, 0.35 mmol/L; glutamate, 0.25 mmol/L. H, Ex vivo FACS analysis of CD8+ <t>splenocyte</t> cell survival (left) and activation (right) following supplementa- tion with 1 mmol/L ammonia (Amn; ref. 59; measured plasma ammonia levels 1.34 mmol/L; n = 5, Student t test); P = 0.011, 0.043, respectively. I, Heat map for differential gene expression in hepatocytes demonstrates a unique pattern on day 21 in the livers of 4T1 breast cancer mice compared with day 4. J, 4T1 day 21 vs. 4T1 day 4 pathway enrichment analysis. Each bar shows the fold enrichment of a specific pathway. TCA, tricarboxylic acid.
Complete Rpmi1640 Culture Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/complete rpmi1640 culture medium/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
complete rpmi1640 culture medium - by Bioz Stars, 2026-02
90/100 stars
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Figure 1. (Continued) G, XTT assay for 4T1 cancer cell proliferation following supplementation of the medium with UC intermediates (n = 3, two-way ANOVA). P values: 24 hours: aspartate: not significant, ammonia = 0.007, glutamate and fumarate <0.0001; 48 and 72 <0.0001 for all measurements. Metabolite concentrations were supplemented in the following concentrations: ammonia, 0.75 mmol/L as published (49); aspartate, 0.25 mmol/L; fuma- rate, 0.35 mmol/L; glutamate, 0.25 mmol/L. H, Ex vivo FACS analysis of CD8+ splenocyte cell survival (left) and activation (right) following supplementa- tion with 1 mmol/L ammonia (Amn; ref. 59; measured plasma ammonia levels 1.34 mmol/L; n = 5, Student t test); P = 0.011, 0.043, respectively. I, Heat map for differential gene expression in hepatocytes demonstrates a unique pattern on day 21 in the livers of 4T1 breast cancer mice compared with day 4. J, 4T1 day 21 vs. 4T1 day 4 pathway enrichment analysis. Each bar shows the fold enrichment of a specific pathway. TCA, tricarboxylic acid.

Journal: Cancer Discovery

Article Title: Early Infiltration of Innate Immune Cells to the Liver Depletes HNF4α and Promotes Extrahepatic Carcinogenesis

doi: 10.1158/2159-8290.cd-22-1062

Figure Lengend Snippet: Figure 1. (Continued) G, XTT assay for 4T1 cancer cell proliferation following supplementation of the medium with UC intermediates (n = 3, two-way ANOVA). P values: 24 hours: aspartate: not significant, ammonia = 0.007, glutamate and fumarate <0.0001; 48 and 72 <0.0001 for all measurements. Metabolite concentrations were supplemented in the following concentrations: ammonia, 0.75 mmol/L as published (49); aspartate, 0.25 mmol/L; fuma- rate, 0.35 mmol/L; glutamate, 0.25 mmol/L. H, Ex vivo FACS analysis of CD8+ splenocyte cell survival (left) and activation (right) following supplementa- tion with 1 mmol/L ammonia (Amn; ref. 59; measured plasma ammonia levels 1.34 mmol/L; n = 5, Student t test); P = 0.011, 0.043, respectively. I, Heat map for differential gene expression in hepatocytes demonstrates a unique pattern on day 21 in the livers of 4T1 breast cancer mice compared with day 4. J, 4T1 day 21 vs. 4T1 day 4 pathway enrichment analysis. Each bar shows the fold enrichment of a specific pathway. TCA, tricarboxylic acid.

Article Snippet: Cells were resuspended in 2 × 106 cells/mL in splenocyte medium (complete RPMI medium supplemented with 50 μmol/L β-mercaptoethanol, 10% sodium pyruvate and nonessential amino acids) supplemented with 6,000 IU/mL IL2 (Chiron, rhIL2) and seeded in 24-well plates precoated with CD3 (BLG #100302).

Techniques: XTT Assay, Ex Vivo, Activation Assay, Clinical Proteomics, Gene Expression